Thermo Fisher Scientific V5 Tag Recombinant Rabbit Monoclonal Antibody (SY30-01)
V5 태그 단백질 검출용 재조합 토끼 단클론 항체로, Western blot, ICC/IF, IP에 적합합니다. 높은 특이성과 민감도, 일관된 로트 간 재현성을 제공합니다. HEK293 발현 시스템에서 생산되며, 단백질 연구 및 태그 단백질 분석에 유용합니다.
- 카탈로그번호
- MA532053
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific V5 Tag Recombinant Rabbit Monoclonal Antibody (SY30-01)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
| Immunoprecipitation (IP) | 1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY30-01 |
| Immunogen | Synthetic peptide immune sequence: CGKPIPNPLLGLDST |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809347 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the larger rabbit immune repertoire
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when specific antibodies are not available.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5).
It is typically used with all 14 amino acids (GKPIPNPLLGLDST), though a shorter 9 amino acid sequence (IPNPLLGLD) is also used.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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