Thermo Fisher Scientific Phospho-Smad5 (Ser463,Ser465) Recombinant Rabbit Monoclonal Antibody (SY09-03)
Smad5 단백질의 Ser463/Ser465 인산화 상태를 검출하는 Thermo Fisher recombinant rabbit monoclonal antibody입니다. Western blot, IHC, ICC/IF에 사용 가능하며 높은 특이성과 재현성을 제공합니다. 인체, 마우스, 랫트 반응성.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY09-03 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser463 and 465 of human Smad5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809348 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, and individual clones are screened to select optimal candidates.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
SMADs are intracellular proteins essential in signaling pathways of serine/threonine kinase receptors in the TGF-β superfamily.
SMADs are classified as:
- Receptor-regulated SMADs (R-SMADs): SMAD1, 2, 5, 8, 9
- Common-mediator SMAD (Co-SMAD): SMAD4
- Inhibitory SMADs (I-SMADs): SMAD6, 7
SMAD1, 5, 8, and 9 share high sequence homology, and antibodies recognizing common sequences are available. SMAD8 and SMAD9 are often used interchangeably in literature.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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