Thermo Fisher Scientific YY1 Recombinant Rabbit Monoclonal Antibody (SY29-01)
Recombinant rabbit monoclonal antibody targeting YY1 transcription factor. Validated for WB, IHC, ICC/IF, and ChIP applications. High specificity and consistency with animal-free formulation. Suitable for human, mouse, and rat samples.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY29-01 |
| Immunogen | Synthetic peptide within Human YY1 aa 255–304 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809346 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to rabbit immune repertoire
Target Information
YY1 is a ubiquitously distributed transcription factor of the GLI-Kruppel zinc finger protein family. It functions in both activation and repression of various promoters. YY1 may recruit histone deacetylases or acetyltransferases to modulate promoter activity, implicating histone modification in its regulatory mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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