Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Red 685

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.8 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• Request custom conjugation

Product Specific Information

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6).
Mouse Ly108 (also known as NTB-A) is expressed on NK, T, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and IFN-γ expression, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease.
CD4+ T cells from Ly108 knock-out mice exhibit diminished IL-4 production and defective neutrophil functions.

Staining has not revealed strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes and thymocytes (≤ 0.8 µg/test, 100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).
Careful titration recommended for optimal performance.

NovaFluor dyes are incompatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains with NovaFluor dyes.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures minimizing spillover and spread.

Excitation: 637 nm
Emission: 685 nm
Laser: 633–640 nm (Red)

Target Information

Ly108 is the mouse homologue of human SLAMF6.
Expressed on NK, B, and T cells.
Activation enhances NK cell activity and IFN-γ expression.

For Research Use Only. Not for use in diagnostic procedures.

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