Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Blue 660-120S, eBioscience

Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Blue 660-120S

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: ≤ 0.8 µg/test

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 610 / 660 / 700 / 755
• PE
• Request custom conjugation available

Product Specific Information

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6).
Ly108, also known as NTB-A, is expressed on NK, T, and B cells. Engagement of Ly108 enhances NK cell activity and IFN-γ expression, and is implicated in X-linked lymphoproliferative disease.
CD4⁺ T cells from Ly108 knockout mice show reduced IL-4 production and defective neutrophil functions.

• No strain differences observed between C57BL/6 and BALB/c mice.
• Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.
• Reported for use in flow cytometric analysis of mouse splenocytes and thymocytes.
• Recommended usage: ≤ 0.8 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
• Carefully titrate for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining with NovaFluor conjugates.
  • Add 5 µL per sample (regardless of NovaFluor count).
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, with engineered fluorescent signatures minimizing spillover and spectral spread.

• Excitation: 509 nm
• Emission: 665 nm
• Laser: 488 nm (Blue)

Target Information

Ly108 is the mouse homologue of human SLAMF6, expressed on NK, B, and T cells. Activation enhances NK cell activity and IFN-γ expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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