Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Red 685, eBioscience

Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), NovaFluor Red 685

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: ≤ 0.8 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• Biotin
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• Custom Conjugation Request Available

Product Specific Information

The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6).
Ly108, also known as NTB-A, is expressed on NK, T, and B cells. Engagement of Ly108 enhances NK cell activity and IFN-γ expression and is involved in X-linked lymphoproliferative disease mechanisms.

No strain differences were observed between C57BL/6 and BALB/c mice.
Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse splenocytes and thymocytes. Recommended ≤ 0.8 µg/test (100 µL final volume). Cell number may vary (10⁵–10⁸ cells/test). Titrate for optimal performance.

Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assays.

CellBlox Plus Blocking Buffer:
Updated conjugates ship with CellBlox Plus Blocking Buffer (Cat. No. C001T06F01).
Add 5 µL per sample (10³–10⁸ cells) for optimal staining. Use in antibody cocktails prior to mixing with cells.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for improved spectral separation and minimized spillover.

Spectral Characteristics:

• Excitation: 637 nm
• Emission: 685 nm
• Laser: 633–640 nm (Red)

Target Information

Ly108 is the mouse homologue of human SLAMF6.
Expressed on NK, B, and T cells; activation enhances NK cell activity and IFN-γ expression.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without authorization.

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