Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), FITC, eBioscience

Thermo Fisher Scientific CD34 Monoclonal Antibody (4H11), FITC, eBioscience™

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600
• Super Bright 645
• Custom conjugation available upon request

Product Specific Information

The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family that includes endoglycan and podocalyxin, characterized by single-pass transmembrane proteins with a heavily glycosylated extracellular mucin domain.
CD34 is expressed on hematopoietic progenitors and used widely as a marker for stem cell isolation. It is also found on mesenchymal stem cells, tumor cell lines, and vascular endothelium.
Epitopes of CD34 are classified into three classes (I, II, III) based on enzyme sensitivity; 4H11 belongs to class III, reacting with a protein epitope.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and validated in flow cytometry using normal human peripheral blood cells. Recommended usage is 5 µL (0.5 µg) per test in 100 µL final volume.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD34 is a highly glycosylated monomeric surface protein (111–115 kDa) expressed on various stem cell populations. It serves as a stem cell marker and may function as a receptor regulating adhesion, differentiation, and proliferation.
CD34 expression reflects a specific hematopoietic development state, potentially mediating attachment to bone marrow extracellular matrix or stromal cells.
It may act as a scaffold for lineage-specific glycans, facilitating interactions with lectins or selectins.
Phosphorylation by activated protein kinase C suggests a role in signal transduction.
Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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