Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), FITC, eBioscience
인간 CD31(PECAM-1)을 인식하는 FITC 결합 단클론 항체로, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 내피세포 표지 및 세포 간 상호작용 연구에 사용됩니다. 488 nm 여기, 520 nm 방출 특성을 가지며, 4°C 암소 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 7 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 43 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Baboon, Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | WM-59 (WM59) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2043836 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- eFluor 450
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The WM59 monoclonal antibody reacts with human CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells and at low levels on leukocytes and platelets. CD31 is involved in adhesion, cell-cell and cell-matrix interactions, and signal transduction. CD38 has been reported to bind CD31.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated and tested by flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per test in 100 µL final volume. Cell number: 10⁵–10⁸ cells/test.
Excitation: 488 nm
Emission: 520 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs, providing docking sites for protein-tyrosine phosphatases upon phosphorylation. It is expressed throughout the vascular system, mainly at cell junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
CD31 plays roles in:
- Integrin-mediated cell adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immunoreceptor signaling
- Autoimmunity and thrombosis
For Research Use Only. Not for diagnostic procedures or resale without authorization.
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