Thermo Fisher Scientific AKT2 Monoclonal Antibody (16G11.E8), APC
AKT2 단백질을 인식하는 Rat 모노클로날 항체로, APC 형광 표지되어 있습니다. Western blot, IHC, Flow cytometry 등 다양한 분석에 사용 가능하며, Human AKT2의 인산화 및 비인산화 형태 모두 검출합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific AKT2 Monoclonal Antibody (16G11.E8), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Dot Blot (DB) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16G11.E8 |
| Immunogen | Synthetic peptide corresponding to internal residues of human AKT2 protein |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.5M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- Unconjugated
- Biotin
- FITC
- PE
- Custom conjugation available upon request
Product Specific Information
Store vial at 4°C prior to restoration. Restore with deionized water. Stable for several weeks at 4°C as undiluted liquid. Dilute only before immediate use. Centrifuge if not clear after standing at room temperature. For extended storage, aliquot and freeze at -20°C or below. Avoid freeze/thaw cycles. Store reagent in the dark and handle under subdued lighting.
Anti-AKT2 antibody detects both unphosphorylated and phosphorylated forms of AKT2. Cross-reactivity with mouse and rat is expected due to high sequence identity (mouse 88%, rat 90%).
Target Information
AKT2 is a phosphoinositide-dependent serine/threonine kinase enriched in insulin-responsive tissues. It plays a key role in cellular signaling pathways related to growth factor receptors and PI3-kinase activation. AKT2 amplification and overexpression are observed in certain human cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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