Thermo Fisher Scientific CD40 Monoclonal Antibody (5C3), NovaFluor Yellow 610, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 685
• NovaFluor Red 755
• NovaFluor Yellow 660
• NovaFluor Yellow 690
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The 5C3 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein. CD40, a member of the TNFR family, is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. It regulates B cell development and maturation by inducing Ig isotype-switching and, in combination with IL-4, protects B cells from apoptosis and promotes proliferation.

Interaction of CD40 with CD154 (gp39) on T cells is crucial for T–B cell communication and immune regulation. The 5C3 antibody is commonly used for B cell activation in in vitro functional assays.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested on normal human peripheral blood cells. Use 5 µL (0.6 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., PI, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for best compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before mixing with cells.
  • For single-color controls: 5 µL per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for minimal spillover and optimized spectral performance.
Excitation: 552 nm; Emission: 612 nm; Laser: 561 nm (Yellow).

Target Information

CD40 is a single-chain glycoprotein and a member of the TNF/NGF superfamily, homologous to the Hodgkin’s disease antigen CD30. It is present on all B cells (except plasma cells), epithelial cells, carcinomas, and lymphoid dendritic cells. CD40 is essential for immune and inflammatory responses, including:

• T cell-dependent immunoglobulin class switching
• Memory B cell development
• Germinal center formation

The AT-hook transcription factor AKNA regulates CD40 expression, mediating cell interactions. TNFR2 interacts with CD40 to mediate signal transduction. CD40–CD154 interaction is implicated in amyloid-beta-induced microglial activation, relevant to Alzheimer’s disease pathogenesis.
Two alternatively spliced transcript variants of CD40 are known.
CD40 dysfunction is associated with Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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