Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (C-04), Biotin
Cytokeratin 18 단백질을 인식하는 Biotin 결합 단클론 항체로, 다양한 인체 및 포유류 조직에서 반응. Western blot, IHC, ICC, Flow cytometry 등 다중 응용 가능. 고순도 액상 형태로 안정적이며 연구용으로 적합.
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Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (C-04), Biotin
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1–4 µg/mL |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mammal, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C-04 |
| Immunogen | Cytoskeleton preparation of epidermal carcinoma cell line A431 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075163 |
Additional Formats
Product Specific Information
This antibody reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with cytokeratin 8. It is expressed in epithelial tissues including gastrointestinal, respiratory, and urogenital tracts, as well as endocrine, exocrine, and mesothelial cells.
Cytokeratin 18 is primarily found in non-squamous epithelia and is present in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas. Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been linked to cryptogenic cirrhosis.
Expression patterns of cytokeratin subtypes are increasingly used to distinguish different epithelial malignancies. Cytokeratin antibodies assist in differential diagnosis of tumors via immunohistochemistry and are useful tools in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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