Thermo Fisher Scientific Cytokeratin 18 Monoclonal Antibody (DC-10), Biotin
Cytokeratin 18 단백질을 인식하는 Biotin 결합 단일클론 항체로, Human 시료에 반응합니다. Western blot, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합하며, 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | 8 publications |
| Immunohistochemistry (IHC) | – | 9 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | Assay-dependent | – |
| Flow Cytometry (Flow) | 2–6 µg/mL | 1 publication |
| ELISA | Assay-dependent | 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DC-10 |
| Immunogen | Human breast carcinoma cell line PMC-42 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075161 |
Additional Formats
Product Specific Information
This antibody reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of the intermediate filament subfamily of intracellular proteins, represented in epithelial tissues.
Target Information
Cytokeratin 18 (Keratin 18, KRT18, type I cytoskeletal 18) belongs to the type A (acidic) subfamily of low molecular weight keratins, typically found in combination with cytokeratin 8.
It is expressed in epithelial tissues of the gastrointestinal, respiratory, and urogenital tracts, as well as in endocrine, exocrine, and mesothelial cells.
Cytokeratin 18 is primarily found in non-squamous epithelia and in most adenocarcinomas and ductal carcinomas, but not in squamous cell carcinomas.
Hepatocellular carcinomas express only cytokeratins 8 and 18. Mutations in the cytokeratin 18 gene have been associated with cryptogenic cirrhosis.
Two transcript variants encoding the same protein have been identified for the cytokeratin 18 gene.
Cytokeratin subtype expression patterns are valuable in distinguishing different epithelial malignancies.
Cytokeratin antibodies assist in differential tumor diagnosis via immunohistochemistry and are also useful in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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