Thermo Fisher Scientific ROR gamma (t) Monoclonal Antibody (B2D), APC, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 1 µg/test

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Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein. ROR gamma belongs to the retinoic acid-related orphan receptor (ROR) family, including ROR alpha and ROR beta. These ligand-dependent transcription factors play roles in multiple physiological processes.
ROR gamma is expressed in tissues such as liver, lung, muscle, heart, and kidney. An isoform, ROR gamma t, is expressed exclusively in lymphoid cells, including CD4+CD8+ double-positive thymocytes, Th17 cells, and lymphoid tissue inducer (Lti) cells.

The ROR gamma t isoform differs from ROR gamma by three amino acids at the N-terminus. Therefore, the B2D antibody reacts with both ROR gamma and ROR gamma t isoforms.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by intracellular staining and flow cytometry using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Recommended use ≤ 1 µg/test in 100 µL final volume. Cell numbers may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Excitation: 633–647 nm
Emission: 660 nm
Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

ROR gamma (NR1 Thyroid Hormone-Like receptor) influences thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. It promotes thymocyte survival via Bcl-x(L) expression and is required for lymph node and Peyer’s patch development.
The thymus-specific isoform ROR gamma(t) inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements containing a GGTCA core motif preceded by a 6 bp AT-rich sequence.

Expression has been observed in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and in human skeletal muscle. ESTs have been isolated from human tissues including cancerous and normal samples from head/neck, germ cell, prostate, colon, lung, liver/spleen, and pancreas.

ROR gamma t (RORgt) is an isoform of RORC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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