Thermo Fisher Scientific Phospho-Vimentin (Ser83) Polyclonal Antibody
인산화된 Vimentin(Ser83)을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ELISA에 적합. 인간, 생쥐, 랫트 반응성. 항원 친화 크로마토그래피로 정제, 액상 형태로 제공. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human Vimentin around the phosphorylation site of S83 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.5% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609604 |
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During development, vimentin is exchanged for new, tissue-specific IFPs.
Vimentin has been implicated in steroid synthesis regulation via its role as a storage network for steroidogenic cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase causes intermediate filament breakdown and activation of an ATP and myosin light chain-dependent contractile event. This facilitates cytoskeletal changes that promote lipid droplet interaction with mitochondria, enhancing cholesterol transport and steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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