Thermo Fisher Scientific ICAM3 Monoclonal Antibody (OTI1E7), TrueMAB
ICAM3 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간 및 개 반응성. 동결건조 형태로 제공되며 PBS 버퍼에 보존. 연구용으로 T세포 활성화 및 면역반응 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E7 |
| Immunogen | Full length human recombinant protein of human ICAM3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CD50 (ICAM-3) is a transmembrane glycoprotein expressed by leukocytes. It acts as a counter-receptor for LFA-1 integrin and mediates interactions between T cells and antigen-presenting cells. ICAM-3 regulates LFA-1 affinity for ICAM-1, induces T cell activation and proliferation, and plays a crucial role in initiating immune responses. It also interacts with CD209 (DC-SIGN) on dendritic cells and macrophages, facilitating communication between dendritic cells and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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