Thermo Fisher Scientific PR3 Monoclonal Antibody (WGM2), FITC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (Frozen) (IHC (F))

1:50

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:50

-

Immunoprecipitation (IP)

1:50

-

Functional Assay (Functional)

1:50

-

Inhibition Assays (IA)

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

WGM2

Immunogen

Monoclonal antibody WGM2 reacts with human proteinase 3 (PR3), a 30 kDa protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PR3,uniProtId:P24158-1,ncbiNodeId:9606,antigenRange:1-256,antigenLength:256,antigenImageFileName:MA5-49095_PR3_P24158-1_House_mouse.svg,antigenImageFileNamePDP:MA5-49095_PR3_P24158-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

View additional formats

• Unconjugated
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

100 µg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.02% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Wet ice

RRID

AB_3093254

Product Specific Information

Monoclonal antibody WGM2 blocks the PR3 activity and partially inhibits the binding of human PR3-ANCA to PR3.

Target Information

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed:3198760, PubMed:2033050, PubMed:28240246). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed:23202369). May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed:22266279). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.