Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 1.0 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 563, 661, 737
• Brilliant Violet 480, 605
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue, Red, Violet, Yellow Series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright Series (436–780)
• Request custom conjugation available

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a key role in T cell development and activation. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse splenocytes (≤1.0 µg/test; 100 µL final volume; 10⁵–10⁸ cells/test)
• Recommended: Titrate for optimal performance

Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is intended for use on cytometers equipped with a UV (355 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD4 antigen is involved in MHC class II recognition and serves as a co-receptor for HIV. It is primarily expressed in T helper cells but also found in monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and various non-lymphoid tissues such as brain and gut. CD4 mediates T-cell activation and may contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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