Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Additional Formats

• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, a 32–34 kDa cell surface receptor expressed as a heterodimer (CD8α/β) or homodimer (CD8α/α). CD8a is found on thymocytes, mature T cells, and NK cells. It binds to MHC class I and, through association with p56lck, plays a role in T-cell development and activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg)/test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume (cell count: 10⁵–10⁸ cells/test).

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm; use with UV (355 nm) laser-equipped cytometers.

For panels containing multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates. Powered by Sirigen.

Target Information

CD8 (Cluster of Differentiation 8) is a type I transmembrane glycoprotein functioning as a co-receptor for MHC class I molecules. It is expressed on T cells, NK cells, monocytes, and dendritic cells. CD8α is essential for MHC-I binding and contributes to T-cell activation. Upon antigen recognition, CD8 recruits Lck kinase, leading to cytokine production and cytotoxic T lymphocyte activation. Activated CTLs play a key role in pathogen and tumor cell clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures.

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