Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue 510, 585, 610-30S, 660-40S
• NovaFluor Yellow 570, 590, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM that associates with CD18 to form Mac-1 (αMβ2 integrin). It binds to CD54 (ICAM-1), C3bi, and fibrinogen, and is expressed on macrophages, NK cells, granulocytes, activated lymphocytes, and mouse B-1 cells. M1/70 also cross-reacts with human CD11b for detection on monocytes, granulocytes, and NK cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Mouse bone marrow cells (≤0.25 µg/test)
• Recommended: Titrate antibody for optimal performance

Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for UV (355 nm) laser-equipped cytometers. Confirm instrument compatibility before use.

When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM) is a 165 kDa adhesion molecule forming a heterodimer with CD18 (Mac-1, CR3). It is expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells, dendritic cells, and microglia. CD11b/CD18 mediates adhesion to ICAMs, fibrinogen, and complement components, playing key roles in immune cell interactions.

Expression levels vary by tissue and cell type—higher in peritoneal macrophages than splenic macrophages. CD11b is widely used as a microglial marker and is implicated in lupus-related pathologies.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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