Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), Brilliant Ultra Violet 395, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 0.5 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 563, 615, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 615
• FITC
• NovaFluor Blue 585, 610-70S, 660-120S, 760
• NovaFluor Yellow 590, 610, 660
• Pacific Orange
• PE, PE-Cyanine5, 5.5, 7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays an important role in T cell development and function. In T cells, CD4 associates with protein tyrosine kinase p56lck. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm, excitable by a 355 nm UV laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Excitation: 350 nm
Emission: 395 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD4 is a co-receptor for MHC class II recognition and is also a receptor for HIV. It is expressed on T helper cells and other immune cells such as monocytes, macrophages, and dendritic cells. CD4 expression is found in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues. It plays a role in T-cell activation via association with Lck kinase and may mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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