Thermo Fisher Scientific MBD1 (Methyl-CpG Binding Domain Protein 1) Monoclonal Antibody (CPTC-MBD1-1)

Thermo Fisher Scientific MBD1 (Methyl-CpG Binding Domain Protein 1) Monoclonal Antibody (CPTC-MBD1-1)

Applications

• Immunohistochemistry (Paraffin) (IHC (P))

Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

Target Information

Methyl-CpG-binding domain protein 1 (MBD1) is a member of a family of mammalian proteins that specifically bind methylated regions of DNA.
It acts as a transcriptional repressor that binds CpG islands in promoters where DNA is methylated at position 5 of cytosine within CpG dinucleotides.
Binding is abolished by the presence of 7-mG produced by DNA damage from methylmethanesulfonate (MMS).
MBD1 plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1.
Isoform 1 and isoform 2 can also repress transcription from unmethylated promoters.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.