Thermo Fisher Scientific LSD1 Monoclonal Antibody (K.884.2)
LSD1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, IHC, ICC/IF, IP, ChIP 등 다양한 응용에 적합. 인간, 마우스, 원숭이, 랫트에 반응하며, 고순도 친화 크로마토그래피 정제. 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:800 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| Immunoprecipitation (IP) | 1:50 |
| ChIP assay (ChIP) | 5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | K.884.2 |
| Immunogen | Synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 17 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10984411 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
LSD1 is a histone demethylase that specifically demethylates Lys-4 of histone H3, a marker for epigenetic transcriptional activation, thereby acting as a corepressor. It contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. LSD1 is part of several histone deacetylase complexes and silences genes via histone demethylation. It oxidizes substrates through FAD to generate imines that are hydrolyzed. LSD1 demethylates both mono- and tri-methylated Lys-4 of histone H3 and may repress neuronal genes. It requires RCOR1/CoREST for nucleosomal demethylation and may also demethylate Lys-9 of histone H3, leading to derepression of androgen receptor target genes. Mutations in this gene can cause cleft palate, psychomotor retardation, and distinctive facial features.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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