Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Red 710, eBioscience

Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Red 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 725
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Request custom conjugation

Product Specific Information

The monoclonal antibody 2D3 reacts to CD47, also known as integrin-associated protein (IAP) and neurophilin. CD47 is a glycosylated five-transmembrane protein involved in adhesion via interactions with SIRP and integrins (CD51/CD61, CD41/CD61). It mediates bi-directional signaling affecting neural synaptic activity and macrophage phagocytosis. CD47 acts as a receptor for thrombospondin, influencing T cell activation or apoptosis. Expression is found in hematopoietic cells (T/B cells, monocytes, platelets, erythrocytes) and non-hematopoietic cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested using normal human peripheral blood cells at 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
• Add 5 µL per sample for antibody cocktails or single-color controls.

Technology:
NovaFluor conjugates use Phiton™ technology, based on nucleic acid dye structures engineered for minimal spillover and spread.

Optical Properties:
Excitation: 639 nm
Emission: 710 nm
Recommended Laser: 633–640 nm (Red)

Target Information

CD47 (Integrin-associated protein, IAP) is a widely expressed transmembrane glycoprotein interacting with integrins and regulating signaling pathways, including G protein activation and calcium flux during cell adhesion. It serves as a receptor for thrombospondin and modulates membrane transport and signal transduction. CD47 acts as a ligand for CD172a (SIRPα), preventing macrophage-mediated phagocytosis of CD47-positive cells. It influences cell migration, B cell adhesion, T cell activation, chondrocyte mechanosignaling, and neuronal development—especially abundant in synapse-rich regions of the brain and retina.

For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.

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