Thermo Fisher Scientific CD47 Monoclonal Antibody (2D3), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.6 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Blue 690
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• NovaFluor Yellow 755
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The monoclonal antibody 2D3 reacts with CD47, also known as integrin-associated protein (IAP) and neurophilin.
CD47 is a glycosylated five-transmembrane protein involved in adhesion via interactions with SIRP (signal regulator protein) and beta3 integrins (CD51/CD61 and CD41/CD61). These interactions mediate bi-directional signaling affecting neural synaptic activity and macrophage phagocytosis. CD47 also serves as a receptor for thrombospondin and is expressed in most hematopoietic and non-hematopoietic cells.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported

• Suitable for flow cytometric analysis

Applications Tested

• Pre-titrated and tested on normal human peripheral blood cells
• Recommended use: 5 µL (0.6 µg) per test
• A test is defined as staining in a 100 µL final volume
• Cell number: 10⁵–10⁸ cells/test (empirically determined)

Important Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
• Use Invitrogen LIVE/DEAD Fixable Dead Cell Stains instead.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails before combining with cells.
• For single-color controls: use 5 µL of blocking buffer per 100 µL of cell sample (10³–10⁸ cells).

NovaFluor Technology

NovaFluor conjugates utilize Phiton™ technology, employing novel nucleic acid dye structures engineered to minimize spillover and spread in multicolor flow cytometry.
Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD47 (integrin-associated protein, IAP) is a ubiquitously expressed transmembrane glycoprotein that interacts with several integrins to regulate their functions.
Engagement with ligands or counter receptors modulates signaling pathways, including activation of heterotrimeric G proteins and calcium signaling upon cell adhesion.
CD47 also serves as a receptor for the C-terminal domain of thrombospondin, playing roles in membrane transport, signal transduction, and immune regulation.
The CD47–CD172a (SIRPα) interaction inhibits macrophage-mediated phagocytosis of CD47-positive cells and influences cell migration, adhesion, and neuronal development.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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