Thermo Fisher Scientific CD38 Monoclonal Antibody (14.27), eFluor 660, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The monoclonal antibody 14.27 reacts with rat CD38, also known as ADP-ribosyl cyclase. CD38 is a type II transmembrane glycoprotein and ectoenzyme that catalyzes the synthesis and hydrolysis of cADPR and NAADP, secondary messengers involved in calcium mobilization. CD38 can also act as a receptor for CD31 or agonist monoclonal antibodies.

CD38 is expressed by leukocytes, early hematopoietic precursor cells, epithelial cells, astrocytes, some muscle cells, kidney cells, and thyroid cells. It plays a role in pro-inflammatory cytokine production, proliferation, cell activation, migration, adhesion, and protection from apoptosis.

Applications Reported:
This 14.27 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended usage ≤ 0.25 µg per test (in 100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Fluorophore Information:
eFluor® 660 replaces Alexa Fluor® 647. It emits at 659 nm and is excited with a red laser (633 nm). Ensure the instrument can detect this fluorochrome.

• Excitation: 633–647 nm
• Emission: 668 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD38 (NAD⁺ glycohydrolase) is a type II transmembrane glycoprotein that induces activation, proliferation, and differentiation of mature lymphocytes and mediates apoptosis of myeloid and lymphoid progenitor cells. It functions as a multi-catalytic ectoenzyme (ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, NAD⁺ glycohydrolase) and as a cell surface receptor.

Antibodies to CD38 are useful in:

• Subtyping of lymphomas and leukemias
• Detection of plasma cells (e.g., myeloma identification)
• Marker for activated B and T cells

CD38 participates in cell adhesion, signal transduction, and calcium signaling, and is expressed in pancreas, liver, kidney, malignant lymphoma, and neuroblastoma.
Diseases associated with CD38 dysfunction include chronic lymphocytic leukemia and Richter’s Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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