Thermo Fisher Scientific bNOS Polyclonal Antibody
Thermo Fisher Scientific의 bNOS Polyclonal Antibody는 인간 및 랫트 반응성을 가진 고품질 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 안정적인 PBS 버퍼에 보관됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific bNOS Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the internal segment of human bNOS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Human fetal brain lysate, liver, retina
Target Information
NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH, and O₂) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme).
Several distinct NOS isoforms have been described and shown to represent the products of three distinct genes. These include two constitutive Ca²⁺/CaM-dependent forms of NOS:
- NOS1 (ncNOS): activity first identified in neurons
- NOS3 (ecNOS): first identified in endothelial cells
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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