Thermo Fisher Scientific Tubulin beta Polyclonal Antibody
Rabbit polyclonal antibody against human beta-tubulin for WB, IHC, and IP applications. Recognizes cytoplasmic tubulin with high specificity. Suitable for use as a loading control in protein detection assays. Supplied as liquid, 0.5 mg/mL, stored at 4°...
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Thermo Fisher Scientific Tubulin beta Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 2–10 µg/mL |
| Immunoprecipitation (IP) | 2–5 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to aa 416–430 of human tubulin beta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: HeLa cells, Lung tissue
- Cellular location: Cytoplasmic
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are associated with congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells and transiently in non-neuronal embryonic tissues. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, inhibiting its incorporation into microtubules.
Microtubules, composed of alpha and beta tubulin dimers (~55 kDa each), are major cytoskeletal elements essential for chromosome segregation, intracellular transport, ciliary and flagellar movement, and structural support. Beta-tubulin is widely used as a loading control in protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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