Thermo Fisher Scientific CYP3A5 Monoclonal Antibody (F18 P3 B6)
CYP3A5 단백질을 인식하는 Mouse monoclonal 항체로, WB, IHC, ICC, ELISA에 사용 가능. Human 및 Bovine 시료에서 반응하며 약물 대사 연구에 적합. 액상 형태로 제공되며 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 2 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:250 | View 1 publication |
| ELISA | Assay-dependent | - |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Human |
| Published Species | Human, Non-human primate |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F18 P3 B6 |
| Immunogen | Synthetic peptide corresponding to residues E S R D G T L S G E, conjugated to ovalbumin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2090513 |
Product Specific Information
MA3-033 detects Cytochrome P450 3A5 from HEK293 and HepG2 human cell lines, and from bovine liver and pancreas samples.
It has been successfully used in Western blot applications, detecting a ~54 kDa band corresponding to Cytochrome P450 3A5.
The immunogen is a synthetic peptide corresponding to residues E S R D G T L S G E, conjugated to ovalbumin.
Target Information
CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. These monooxygenases catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids.
This protein localizes to the endoplasmic reticulum, and its expression is induced by glucocorticoids and some pharmacological agents.
The enzyme metabolizes drugs such as nifedipine and cyclosporine, as well as steroid hormones including testosterone, progesterone, and androstenedione.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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