Thermo Fisher Scientific uNOS Monoclonal Antibody (NOS-3F7-B11 B5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50-1:500

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 8 publications 8 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:20

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

NOS-3F7-B11 B5

Immunogen

Purified bovine bNOS.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325476

Product Specific Information

MA3-030 detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, human, mouse and rat tissues.

MA3-030 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and immunohistochemical experiments. By Western blot, this antibody detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS. Immunohistochemical staining of NOS with MA3-030 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.

The MA3-030 antigen is purified bovine bNOS.

Target Information

NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.