Thermo Fisher Scientific GSK3 alpha/beta Monoclonal Antibody (4G-1E)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

-

Flow Cytometry (Flow)

1:100

-

Immunoprecipitation (IP)

Assay-Dependent

-

RNA Immunoprecipitation (RIP)

Assay-dependent

-

Product Specifications

Species Reactivity

Dog, Fruit fly, Human, Mouse, Non-human primate, Rat

Published species

Fruit fly, Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4G-1E

Immunogen

Synthetic peptide corresponding to residues 203-219 of drosophila GSK-3 coupled to ovalbumin: KQLLHGEPNVSYICSRY if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GSK3 alpha/beta,uniProtId:P83101-1,ncbiNodeId:7227,antigenRange:203-219,antigenLength:501,antigenImageFileName:MA3-038_GSK3_alphabeta_P83101-1_House_mouse.svg,antigenImageFileNamePDP:MA3-038_GSK3_alphabeta_P83101-1_House_mouse_PDP.jpeg,sortOrder:4}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2608651

Product Specific Information

MA3-038 detects GSK3 alpha/beta in Human, Mouse, Rat Canine, and Non-human primate samples.

Immunogen is a synthetic peptide corresponding to residues 203-219 of drosophila GSK-3, which is highly conserved across multiple species.

Target Information

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimers disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.