Thermo Fisher Scientific TRADD Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 199-312 of human TRADD (NP_0037801) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRADD,uniProtId:Q15628-1,ncbiNodeId:9606,antigenRange:199-312,antigenLength:312,antigenImageFileName:PA5-88134_TRADD_Q15628-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88134_TRADD_Q15628-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.52 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804678

Product Specific Information

Positive Samples: MCF7, Jurkat; Cellular Location: Cytoplasm, Nucleus, cytoskeleton

Immunogen sequence: AQTFLFQGQP VVNRPLSLKD QQTFARSVGL KWRKVGRSLQ RGCRALRDPA LDSLAYEYER EGLYEQAFQL LRRFVQAEGR RATLQRLVEA LEENELTSLA EDLLGLTDPN GGLA

Target Information

TRADD (Tumor necrosis factor receptor type 1-associated DEATH domain protein) is an important mediator of FAS and TNFR1 associated apoptotic signaling. The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12. TRADD acts by interacting with TRIP12 and leading to the disruption of the interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A. TRADD acts as an adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interation with FADD. Overexpression of TRADD leads to two major TNF-induced responses - apoptosis and activation of NF-kappa-B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.