Thermo Fisher Scientific p47phox Polyclonal Antibody
Thermo Fisher Scientific의 p47phox Polyclonal Antibody는 인간 시료에 반응하는 Rabbit IgG 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. Affinity Chromatography로 정제되었으며 PBS/glycerol buffer에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–390 of human p47phox/NCF1 (NP_0002564) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.59 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804682 |
Product Specific Information
Immunogen sequence:
MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCST LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYL MPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV EGDEVSLLEG EAVEVIHKLL DGWWVIRKDD VTGYFPSMYL QKSGQDVSQA QRQIKRGAPP RRSSIRNAHS IHQRSRKRLS QDAYRRNSVR FLQQRRRQAR PGPQSPGSPL EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV
Positive Samples: THP-1, Raji, HL-60, Mouse spleen, Mouse lung, Mouse liver, Mouse brain
Cellular Location: Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein, cytosol
Target Information
p47phox (NCF1) is required, along with NCF2 and membrane-bound cytochrome b558, for activation of the latent NADPH oxidase. This oxidase is a multicomponent enzyme that produces superoxide anion upon activation. Mutations in the gene can result in granulomatous disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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