Thermo Fisher Scientific EOMES Monoclonal Antibody (Dan11mag), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 39 publications 39 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 10 publications 10 publications

Flow Cytometry (Flow)

Assay-Dependent

View 55 publications 55 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Bat, Human, Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

Dan11mag

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 660
• APC
• eFluor 450
• eFluor 660
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865089

Product Specific Information

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in immunohistochemistry of formalin-fixed paraffin embedded tissue sections, intracellular staining followed by flow cytometric analysis, and western blotting. (Fluorochrome conjugated Dan11mag is recommended for use in intracellular flow cytometry.).

Applications Tested: This Dan11mag antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse splenocytes and/or western blotting at 5 µg/mL. This Dan11mag antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low pH antigen retrieval at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.