Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), eBioscience

Thermo Fisher Scientific FOXP3 Monoclonal Antibody (236A/E7), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488
• APC
• Biotin
• eFluor 450
• eFluor 570
• eFluor 660
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine7
• Custom conjugation available upon request

Product Specific Information

The 236A/E7 antibody reacts with human Foxp3 protein (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3 (49–55 kDa) is a transcriptional regulator from the forkhead/winged-helix family, identified as the defective gene in scurfy mice. High Foxp3 expression is characteristic of CD4+CD25+ regulatory T cells (Treg), and ectopic expression in CD4+CD25– cells induces a Treg phenotype.

Using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523), intracellular staining of human PBMCs shows specific staining of CD4+CD25bright populations.

The epitope recognized by 236A/E7 differs from that of PCH101 (Product #72-5776-40). This antibody also recognizes rhesus macaque, sooty mangabey, and cynomolgus macaque.

Applications Tested:

• Immunoblotting and IHC of formalin-fixed paraffin-embedded tissue using antigen retrieval (low or high pH).
• Recommended concentration: 1–10 µg/mL for WB; ≤5 µg/mL for IHC.
• Titrate for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is stably expressed in CD25+CD4+ regulatory T cells, at low levels in CD4+CD25– cells, and absent in CD4–CD8+ T cells. FOXP3 acts as a master regulatory gene and marker of Treg cells. Defects cause the scurfy phenotype in mice and IPEX syndrome in humans (X-linked autoimmunity-allergic dysregulation). Transcript variants encoding different isoforms have been identified. In human breast and colon cancer, FOXP3 expression is regulated by p53 in response to DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.