Thermo Fisher Scientific FOXP3 Monoclonal Antibody (PCH101), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 36 publications 36 publications

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

View 9 publications 9 publications

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Flow Cytometry (Flow)

-

View 120 publications 120 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Baboon, Cynomolgus monkey, Hamster, Human, Mouse, Non-human primate, Rhesus monkey

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

PCH101

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• Biotin
• eFluor 450
• eFluor 660
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2865085

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in scurfy (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, Product # 11-0048-42 , or RPA-T4, Product # 11-0049-42 , depending on the species) and CD25 (BC96, Product # 17-0259-42).

Applications Reported: This PCH101 antibody has been reported for use in immunoblotting (WB) and immunohistology on frozen and paraffin-embedded tissue sections. Use of the purified format is not recommended for intracellular staining for flow cytometric analysis. For additional information on IHC, please visit the Foxp3 FAQs.

Applications Tested: This PCH101 antibody has been tested by immunoblotting (WB) (1-5 µg/mL) of normal human peripheral blood leukocytes. This PCH101 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.