Thermo Fisher Scientific phospho-Tyrosine Monoclonal Antibody (pY20), eBioscience

Applications and Publications

Western Blot (WB)

• Tested Dilution: Assay-Dependent
• Publications: View 2 publications

Immunocytochemistry (ICC/IF)

• Tested Dilution: —
• Publications: View 2 publications

Flow Cytometry (Flow)

• Tested Dilution: Assay-Dependent
• Publications: View 1 publication

Immunoprecipitation (IP)

• Tested Dilution: Assay-Dependent

Product Specifications

Additional Formats

• eFluor 450
• eFluor 660
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The pY20 monoclonal antibody recognizes phosphorylated tyrosine residues (p-Tyr) on proteins. Phosphorylation of specific tyrosine residues plays a key role in intracellular signaling cascades. Detection of p-Tyr residues is valuable for characterizing and purifying phosphorylated proteins and understanding their associated biochemical pathways.

• Applications Reported: Flow cytometry, immunoprecipitation, western blotting
• Applications Tested: Immunoblotting of mouse spleen cells (untreated or treated with sodium orthovanadate)
• Purity: >90% (SDS-PAGE)
• Aggregation: <10% (HPLC)
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

Tyrosine phosphorylation plays a central role in signal transduction, mitogenesis, cell cycle progression, and oncogenic transformation. Antibodies specific for phosphorylated tyrosine residues are essential tools for studying tyrosine-phosphorylated proteins and related pathways. The fluorescein (FITC) conjugate of clone pY20 is particularly useful in immunohistochemical and immunocytochemical assays where secondary antibody detection may interfere with results.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.