Thermo Fisher Scientific Neurofilament (NF-L) (Neuronal Marker) Monoclonal Antibody (NR-4)
Neurofilament NF-L 단백질을 인식하는 NR-4 클론 단일클론 항체. 다양한 종의 신경세포 표지에 사용 가능하며, IHC, ICC, Flow Cytometry 등 다중 응용에 적합. 고순도의 Protein A/G 정제 항체로 안정적이며 연구용으로 최적화됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 2–4 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Chicken, Human, Pig, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | NR-4 |
| Immunogen | Crude neurofilament preparation from porcine spinal cord |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons and are composed predominantly of three major subunits: NF-L, NF-M, and NF-H.
NF-L (light subunit) has a molecular weight of approximately 70 kDa and plays a key role in maintaining neuronal caliber.
NF-M and NF-H represent the medium and heavy subunits, respectively, with NF-H running at 180–220 kDa on SDS-PAGE gels depending on species.
The expression of these neuronal intermediate filament proteins is both tissue-specific and developmentally regulated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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