Thermo Fisher Scientific Cardiotrophin 1 Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein
SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA

Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Human Cardiotrophin-1. Anti-Human Cardiotrophin-1-specific antibody was purified by affinity chromatography employing an immobilized Human Cardiotrophin-1 matrix.

Sandwich ELISA
To detect Human Cardiotrophin-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Cardiotrophin-1 (500-P101Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Human Cardiotrophin-1.

Western Blot
To detect Human Cardiotrophin-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, detection limit for Recombinant Human Cardiotrophin-1 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF, and IL-11.
Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and induces hypertrophy in cardiac myocytes in vitro.
It is a secreted protein expressed at high levels in myocardium during cardiogenesis and promotes proliferation and survival of embryonic cardiomyocytes, suggesting a role in gp130 activation during cardiac development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.