Thermo Fisher Scientific Cardiotrophin 1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Cardiotrophin-1. Anti-Human Cardiotrophin-1-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human Cardiotrophin-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Cardiotrophin-1 (500-P101) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Cardiotrophin-1.

Western Blot:
To detect hCardiotrophin-1 by Western blot, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hCardiotrophin-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P101BT-1MG will be provided as 2 × 500 µg.

Target Information

Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, signaling through gp130 receptor complexes. gp130 complexes with several receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF, and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and induces hypertrophy in cardiac myocytes in vitro. Expressed at high levels in myocardium during cardiogenesis, CT-1 promotes proliferation and survival of embryonic cardiomyocytes, suggesting its role in cardiac development via gp130 activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.