Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 3 publications 3 publications

Radioimmune Assays (RIA)

-

View 2 publications 2 publications

In vitro Assay (IV)

-

View 3 publications 3 publications

Immunostaining (IS)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human VEGF 165 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:VEGF-165,uniProtId:P15692-13,ncbiNodeId:9606,antigenRange:27-191,antigenLength:395,antigenImageFileName:500-P10-100UG_VEGF-165_P15692-13_Rabbit.svg,antigenImageFileNamePDP:500-P10-100UG_VEGF-165_P15692-13_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929183

Product Specific Information

AA Sequence of recombinant protein: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. Anti-Human VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Human VEGF 165 matrix.

Sandwich ELISA: To detect Human VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human VEGF 165 (500-P10Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human VEGF 165.

Western Blot: To detect Human VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human VEGF 165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.