Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, PeproTech
Rabbit 유래 VEGF-165 폴리클로날 항체로, Human VEGF-165 검출에 적합. Western blot, ELISA 등 다양한 응용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체. 동결건조 형태로 -20°C 보관. 연구용으로만 사용.
- 카탈로그번호
- 500-P10-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific VEGF-165 Polyclonal Antibody, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 2 publications |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
| Neutralization (Neu) | – | View 3 publications |
| Radioimmune Assay (RIA) | – | View 2 publications |
| In vitro Assay (IV) | – | View 3 publications |
| Immunostaining (IS) | – | View 4 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Hamster, Human, Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human VEGF 165 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929183 |
Product Specific Information
Amino Acid Sequence (Recombinant Protein)
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.
Preparation
Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF 165. The antibody was purified by affinity chromatography using an immobilized Human VEGF 165 matrix.
Sandwich ELISA
For detection of Human VEGF 165 by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL. When paired with PeproTech Biotinylated Anti-Human VEGF 165 (500-P10Bt), detection sensitivity reaches 0.2–0.4 ng/well of recombinant VEGF 165.
Western Blot
For detection of Human VEGF 165 by Western blot, use 0.1–0.2 µg/mL. Detection limit: 1.5–3.0 ng/lane under reducing or non-reducing conditions with compatible secondary reagents.
Target Information
The vascular endothelial growth factor (VEGF) family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PlGF. VEGF and its receptors are fundamental regulators of angiogenesis signaling. Most tumors require angiogenesis, and VEGF is the most potent angiogenic cytokine involved.
Five isoforms of VEGF result from alternative splicing of a single VEGF gene, binding primarily to tyrosine kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), expressed mainly on endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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