Thermo Fisher Scientific Phospho-p38 MAPK alpha (Thr180) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of p38 MAPK only when phosphorylated at Threonine 180.

Target Information

MAPK14 (p38 alpha) is a member of the stress-activated protein kinase class of MAPKs. The p38 MAPK cascade regulates various cellular responses to stress, inflammation, and other signals. p38 MAPK is relatively inactive in its nonphosphorylated form and becomes rapidly activated by dual phosphorylation of a Thr-Gly-Tyr motif.

There are four isoforms of p38 MAPK, differing in tissue expression and affinity for upstream activators and downstream effectors. When cells are exposed to stimuli such as tumor necrosis factor, interleukin-1, or heat shock, activation of MAPK kinase-3 occurs via phosphorylation. Activated MAPK kinase-3/6 phosphorylate Thr180 and Tyr182 residues in p38 MAPK. Phospho-p38 MAPK subsequently activates transcription factors such as ATF-2, CHOP-1, and MEF-2 through phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.