Thermo Fisher Scientific alpha Synuclein Monoclonal Antibody (3C11), FITC
인간, 마우스, 랫트에서 반응하는 알파 시뉴클레인 단클론 항체로 FITC 형광 표지. WB, IHC, ICC, ELISA 등 다양한 응용 가능. Protein G 정제, 4°C 암소 보관. 파킨슨병 및 신경퇴행성 질환 연구에 적합.
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Thermo Fisher Scientific alpha Synuclein Monoclonal Antibody (3C11), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:1,000 |
| Dot blot (DB) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3C11 |
| Immunogen | Human alpha synuclein monomer (aa 1–140) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932292 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-45838 was sufficient for detection of Alpha Synuclein in 15 µg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Binds within the 81–130 aa region of human alpha synuclein, determined using WB and ELISA with corresponding peptides.
Detects approximately 14 kDa band corresponding to Alpha synuclein. Band at approximately 30 kDa represents a dimer.
Target Information
Alpha-synuclein is a member of the synuclein family, which includes beta- and gamma-synuclein. These proteins are abundant in the brain and play roles in synaptic transmission and membrane trafficking.
Alpha- and beta-synuclein selectively inhibit phospholipase D2, indicating involvement in signaling pathways.
Defects in the SNCA gene, encoding alpha-synuclein, are implicated in Parkinson’s disease.
Alpha-synuclein peptides are major components of amyloid plaques in Alzheimer’s disease.
Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene.
Diseases associated with SNCA include Parkinson Disease 1 (Autosomal Dominant) and Lewy Body Dementia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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