Thermo Fisher Scientific alpha Synuclein Monoclonal Antibody (10H7), APC

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

ELISA (ELISA)

1:1,000

-

Dot blot (DB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10H7

Immunogen

Mouse alpha synuclein aggregate if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:alpha Synuclein,uniProtId:O55042-1,ncbiNodeId:10090,antigenRange:1-140,antigenLength:140,antigenImageFileName:MA5-45840_alpha_Synuclein_O55042-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45840_alpha_Synuclein_O55042-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

APC APC APC

View additional formats

• FITC
• PerCP
• Request custom conjugation

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932294

Product Specific Information

A 1:1,000 dilution of MA5-45840 was sufficient for detection of Alpha Synuclein in 15 µg of human brain cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Binds within the 101-130 aa region of human alpha synuclein, determined using Dot blot and ELISA with corresponding peptides. Detects approximately 14 kDa band corresponding to Alpha synuclein. Band at approximately 40 kDa is a trimer. Does not detect truncated alpha Synuclein (aa 1-114)

Target Information

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. These proteins are abundant in the brain and play a role in regulating synaptic transmission and membrane trafficking. Alpha- and beta-synuclein have been shown to selectively inhibit phospholipase D2, suggesting that they may also be involved in signaling pathways. Defects in the SNCA gene, which encodes alpha-synuclein, have been implicated in the pathogenesis of Parkinsons disease. Additionally, alpha-synuclein peptides are a major component of amyloid plaques in the brains of patients with Alzheimers disease. Multiple alternatively spliced transcripts encoding different isoforms have been identified for this gene. Diseases associated with SNCA include Parkinson Disease 1, Autosomal Dominant and Dementia, Lewy Body.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.