Thermo Fisher Scientific GPR182 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Percent identity with other species by BLAST analysis:
  Human, Gorilla, Gibbon, Monkey (100%); Marmoset, Bovine, Horse (89%); Elephant, Rabbit, Pig (83%)
• For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer.
  After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

Adrenomedullin (ADM) is a hypotensive peptide that belongs to a peptide superfamily, including calcitonin gene-related peptide (CGRP) and amylin. ADM was originally identified in the adrenal medulla, where it is highly expressed. It is also produced by most contractile cells and is upregulated during sepsis and ischemia.
Three distinct receptors can bind ADM: ADM receptor (L1), RDC-1, and the Calcitonin Receptor-Like Receptor (CRLR). The CRLR associates with receptor activity-modifying proteins (RAMPs), determining binding specificity.
Co-expression with RAMP1 results in CRLR binding to CGRP, whereas association with RAMP2 or RAMP3 results in ADM binding. The ADM receptor, a seven transmembrane G protein-coupled receptor, specifically binds ADM and is highly expressed in heart, brain, skeletal muscle, the immune system, adrenal gland, and liver. The ADM receptor mediates ADM signaling through G proteins, which activate adenylate cyclase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.