Thermo Fisher Scientific IL-8 (CXCL8) Polyclonal Antibody, PeproTech
Human IL-8(CXCL8)에 특이적인 Rabbit Polyclonal Antibody로, Western blot과 ELISA에서 높은 감도와 특이성을 제공. 항원 친화 크로마토그래피로 정제되었으며, 무보존 PBS 버퍼에 동결건조 형태로 제공. 염증 및 세포신호 연구에 적합.
- 카탈로그번호
- 500-P28-xxx (3개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific IL-8 (CXCL8) Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | 2 |
| ELISA | 0.5–2.0 µg/mL | 9 |
| Immunoprecipitation (IP) | – | 1 |
| Neutralization (Neu) | – | 5 |
| Blocking Assay (BLOCK) | – | 1 |
| In vitro Assay (IV) | – | 7 |
| Immunostaining (IS) | – | 4 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human IL-8 (CXCL8) (72 a.a.) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930233 |
Product Specific Information
AA Sequence of recombinant protein:
SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-8 (CXCL8). Anti-Human IL-8 (CXCL8)-specific antibody was purified by affinity chromatography employing an immobilized Human IL-8 (CXCL8) matrix.
Sandwich ELISA:
To detect Human IL-8 (CXCL8) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-8 (CXCL8) (500-P28Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human IL-8 (CXCL8).
Western Blot:
To detect Human IL-8 (CXCL8) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human IL-8 (CXCL8) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor belonging to the CXC subfamily of chemokines, bound by cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. Its expression and secretion are induced by diverse inflammatory stimuli in macrophages and endothelial cells. In endothelial cells, IL-8 is stored in Weibel-Palade bodies.
IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg) and signals through CXCR1 and CXCR2 receptors. Previous names include neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP), and protein 3-10C.
IL-8 and ten other CXC chemokines form a gene cluster mapped to chromosome 4q. Cancer studies have shown IL-8’s role in angiogenesis and tumor growth, and it is implicated in bronchiolitis pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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