Thermo Fisher Scientific TIMP1 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TIMP-1. Anti-Human TIMP-1-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hTIMP-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TIMP-1 (500-P280) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hTIMP-1.

Western Blot:
To detect hTIMP-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hTIMP-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P280BT-1MG is provided as 2 × 500 µg.

Target Information

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that forms one-to-one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor.
TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16.
It also functions as a growth factor that regulates cell differentiation, migration, and cell death, and activates cellular signaling cascades via CD63 and ITGB1.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 없음 — OCR 텍스트 통합 완료)