Thermo Fisher Scientific p16INK4a-TAT Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
EPAAGSSMEP SADWLATAAA RGRVEEVRAL LEAGALPNAP NSYGRRPIQV MMMGSARVAE LLLLHGAEPN CADPATLTRP VHDAAREGFL DTLVVLHRAG ARLDVRDAWG RLPVDLAEEL GHRDVARYLR AAAGGTRGSN HARIDAAEGP SDIPDGYGR KKRRQRRR.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human p16-INK4a-TAT. Anti-Human p16-INK4a-TAT-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human p16-INK4a-TAT by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human p16-INK4a-TAT (500-P284) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human p16-INK4a-TAT.

Western Blot:
To detect Human p16-INK4a-TAT by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human p16-INK4a-TAT is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P284TBT-1MG will be provided as 2 × 500 µg.

Target Information

p16-INK4a is a nuclear protein that regulates the cell cycle by inhibiting cyclin-dependent kinase-4 (CDK4) and CDK6. It suppresses tumor formation and induces replicative senescence in various normal cells, including stem cells. Expression increases with age and accumulates in stem cell compartments. Mutations or deletions of the p16-INK4a gene are associated with melanomas and other cancers. TAT fusion proteins provide an alternative method for introducing transcription factors and nuclear proteins into cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.