Thermo Fisher Scientific ICAM-1 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
QTSVSPSKVI LPRGGSVLVT CSTSCDQPKL LGIETPLPKK ELLLPGNNRK VYELSNVQED SQPMCYSNCP DGQSTAKTFL TVYWTPERVE LAPLPSWQPV GKNLTLRCQV EGGAPRANLT VVLLRGEKEL KREPAVGEPA EVTTTVLVRR DHHGANFSCR TELDLRPQGL ELFENTSAPY QLQTFVLPAT PPQLVSPRVL EVDTQGTVVC SLDGLFPVSE AQVHLALGDQ RLNPTVTYGN DSFSAKASVS VTAEDEGTQR LTCAVILGNQ SQETLQTVTI YSFPAPNVIL TKPEVSEGTE VTVKCEAHPR AKVTLNGVPA QPLGPRAQLL LKATPEDNGR SFSCSATLEV AGQLIHKNQT RELRVLYGPR LDERDCPGNW TWPENSQQTP MCQAWGNPLP ELKCLKDGTF PLPIGESVTV TRDLEGTYLC RARSTQGEVT RKVTVNVLSP RYE.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human ICAM-1. Anti-Human ICAM-1-specific antibody was purified by affinity chromatography employing an immobilized Human ICAM-1 matrix.

Sandwich ELISA:
To detect Human ICAM-1 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human ICAM-1 (500-P287Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human ICAM-1.

Western Blot:
To detect Human ICAM-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human ICAM-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

ICAM-1 (CD54) is an 85–110 kDa single-chain type I integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region, and a cytoplasmic domain. ICAM-1 has seven potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102).
ICAM-1 binds to integrins of type CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor.
It is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B and T lymphocytes (low), monocytes, macrophages, dendritic cells, and neutrophils. Expression levels increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in plasma and elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.