Thermo Fisher Scientific UVRAG Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the mid-protein of human UVRAG (C-SRSTIKDNINDK) (aa 391-402). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UVRAG,uniProtId:Q9P2Y5-1,ncbiNodeId:9606,antigenRange:391-402,antigenLength:699,antigenImageFileName:PA5-77811_UVRAG_Q9P2Y5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77811_UVRAG_Q9P2Y5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2736776

Product Specific Information

A 1:1,000 dilution of PA5-77811 was sufficient for detection of UVRAG on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|Predicted molecular weight at approximately 78.1kDa. Observed molecular weights in the 75-90kDa range.

Target Information

UVRAG is predicted to contain a coiled-coil structure and is likely to be metabolically unstable based on the occurrence of a strong PEST domain. It interacts with Beclin 1, leading to activation of autophagy and thereof inhibition of tumorigenicity of human colon cancer cells. The gene for UVRAG protein is localized on human chromosome 11q13. 5 and complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells. The gene has been extremely well conserved during evolution, implying an important role for this gene product in cell metabolism. Chromosomal aberrations involving this gene are associated with left-right axis malformation, and mutations in this gene have been associated with colon cancer. The protein is highly expressed in human brain, lung, kidney and liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.