Thermo Fisher Scientific TCF7 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the C-terminal of Human TCF7 (284-384 aa), conjugated to Keyhole Limpet Haemocyanin (KLH) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TCF7,uniProtId:P36402-1,ncbiNodeId:9606,antigenRange:284-384,antigenLength:384,antigenImageFileName:PA5-77813_TCF7_P36402-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77813_TCF7_P36402-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2736689

Product Specific Information

A 1:1,000 dilution of PA5-77813 was sufficient for detection of TCF7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects 42 kDa, additional band at approximately 54 kDa is another isoform.

Target Information

TCF7 (transcriptional factor 7) is a transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ and CD8+ immature thymocytes. Isoforms for TCF7 lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. TCF7 binds to the T-lymphocyte-specific enhancer element (5-WWCAAAG-3) found in the promoter of the CD3E gene. It may also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7 and CTNNB1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.